幼鼠颌下腺IP_3受体免疫电镜观察

ELECTRON MICROSCOPE IMMUNOGOLD LABELLING OF IP_3 RECEPTOR IN DEVELOPING RAT SUBMANDIBULAR GLAND

目的 :观察 IP3受体在幼年大鼠颌下腺的分布 ,探讨其增龄变化及其意义。方法 :采用胶体金标免疫电镜观察 IP3受体在幼年大鼠颌下腺的分布。结果 :金标颗粒定位于大鼠颌下腺腺泡细胞内质网上 ,以及肌上皮细胞和内皮细胞。 1d龄幼鼠颌下腺 IP3受体免疫金标数量最多 ,随增龄而减少。结论 :IP3受体可能在幼鼠颌下腺细胞的分化。

Objective:To study the localization and significanc e of IP 3 R in submandibular gland of newborn rat Methods:The immunogold labellin g localization of IP\-3R was observed in developing rat submendibular gland Results:The immunogold labelling particles can be seen in the ER of acini cells, myoepithelial and endothelial cells of rat submandibular gland.The immuoreactivity of IP 3R in one-day rat was the most intensive,and it decreases with the aging Concl usion:The IP 3 R may play a role in the development and differentiation o f the cells in developing rat submandibular gland