胆固醇氧化酶转化蛋黄胆固醇工艺的优化

Optimization of Enzymatic Bioconversion of Yolk Cholesterol

研究了用响应面分析法 (RSM )优化酶法转化蛋黄胆固醇的工艺 ,发现胆固醇氧化酶(COD)添加量及蛋黄粉的稀释率是影响蛋黄胆固醇转化率最重要的因素 .在模拟优化的条件下 :反应时间为 14.15h ,蛋黄粉稀释率为 3.5 4 ,COD为 5 .39U /g时 ,胆固醇转化率达 85 6 1% .对氧化产物进行分析发现 :仅有单一产物胆甾烯酮 ,该产物具有降血脂、减肥等功效 .

An efficient enzymatic process for reducing yolk using Response Surface Methodology(RSM) was developed in this paper. In which COD concentration and dilution rate were found to be the most important factors influencing cholesterol conversion. Under the optimum operational conditions: incubation time of 14.15 h, water/solid ratio of 3.54 and COD concentration of 5.39 U/g, about 85.61% yolk cholesterol was reduced. After TLC analysis of the converted product, cholestenone was the only steroid like compound remaining, which was a potential anti obesity and anti hyperlipemia medicine.