草履虫的类中间纤维 以免疫学方法进行的初步研究

Intermediate-like Filaments in ParameciumA Preliminary Investigation with Immunological Methods

以抗中间纤维蛋白抗体作为探针进行免疫荧光实验 ,得到细胞内不同部位的阳性反应 ,暗示原生质中可能存在类中间纤维。这些同源蛋白的胞内分布具有一定的规律性。进一步采用SDS PAGE和免疫印迹技术研究它们的生化性质 ,发现 4种主要蛋白明显有别于胞内其他蛋白组分。它们是 2 1,2 3,33及 68kD蛋白。在免疫印迹实验中 ,4种蛋白中的一种或几种分别与不同的中间纤维蛋白抗体有交叉反应。以上实验初步表明 ,草履虫细胞内存在高等生物中间纤维蛋白的同源蛋白。

Anti-IF-protein antibodies were used as probes for immuno-fluorescence,and the reactions of them with different parts of the cell were detected,which suggested the possibility of the existence of the intermediate-like filaments in the cytoplasm.Those proteins homologous to the antibodies distributed regularly in the protoplasm.To characterize the corresponding proteins,SDS-PAGE and immunoblots were utilized.The 21,23,33 and {68*!kD} proteins were distinguished among the diverse protein constituents of the cell.Some of these proteins also showed the cross-reactivities with anti-IF-proteins antibodies derived from higher organisms.These two evidences both contributed to the homology of some proteins in Paramecium to the intermediate filament proteins in higher organisms.