不同保存液对兔门静脉收缩力的影响

CONTRACTION OF THE RABBIT PORTAL VEIN IN THE PRESENCE AND ABSENCE OF ENDOTHELIUM AND THE PROTECTIVE EFFECT OF UW SOLUTION

将去除和保留内皮细胞的门静脉 ,分别置于 4℃的 UW( U niversity of Wisconsin)液和 Krebs液中保存 ,在 36℃ Krebs液和去甲肾上腺素作用条件下观察不同保存时间对门静脉收缩力的影响 .结果表明 :去除内皮细胞的门静脉 ,无论是在 Krebs液中还是在 UW液中保存 ,其收缩力均随保存时间的延长而下降 ,72 h后分别为 30 %和 6 5 % ;而保留内皮细胞的门静脉 ,其收缩力均随保存时间的延长而增大 ,Krebs液中保存优于 UW液 ,72 h后分别为 2 1 3%和 1 1 8% .无论有无内皮细胞 ,门静脉在 UW液和 Krebs液中保存 ,其收缩力以 UW液中保存的最接近刚离体时的水平 .

University of Wisconsin solution (UW) has been generally used to preserve whole liver up to 24 hours and the preservation efficacy of UW has been confirmed at the hepatocellular level. In this experiment the rabbit portal veins with and without endothelium were suspended in an organ chamber to measure contractile force. After various incubation periods (4 ℃) in Krebs solution or UW solution, veins were treated with norepinephrine (l0 -4 to 10 -5 mol·L -1 , 36 ℃, 95% O 2 and 5% CO 2). Veins without endothelium contracted more after 24, 48, and 72 hours of treatment with UW (84%, 83%, and 65% of control) than after treatment with Krebs solution alone (56%, 44%, and 31%). Veins with endothelium contracted less after 24, 48, and 72 hours of treatment with UW ( 1 380 /0, 1 190 /0, and 1 180 /0) than after treatment with Krebs solution alone (207%, 237%, and 213%). These results indicated that UW preserved the original contractile force of portal vein with and without endothelium better than Krebs solution.