伪狂犬病病毒闽A株gE基因去信号肽片段的克隆与序列测定

Cloning and Sequencing of gE Gene Encoding Area of Pseudorabies Virus Fa Strain Excluding Signal Peptide

根据已经发表的伪狂犬病病毒 (PRV) Rice株的 g E基因序列 ,设计并合成了 1对引物 ,通过 PCR方法扩增到了PRV闽 A株糖蛋白 g E基因除信号肽以外的全部编码区段 ,并克隆到 p MD18- T载体中 ,转化大肠杆菌 XL1- blue菌株。重组质粒 p MD18- T- FL经酶切和 PCR鉴定证实后 ,进行了序列测定。结果表明 ,重组质粒 p MD18- T- FL含有PRV闽 A株糖蛋白 g E基因除信号肽以外的全部编码区段 ,长 16 74bp。序列比较分析表明 ,此区段与 PRV Rice株相应区段的核苷酸序列同源性为 97.5 % ,氨基酸序列同源性为 94.8%

The gE gene encoding area of pseudorabies virus Fa strain excluding signal peptide was amplified by PCR from virus genome DNA.This PCR product was cloned into pMD18-T vector and then sequenced.The sequencing result showed that the recombinant plasmid contained the gE gene encoding area of PRV Fa strain excluding signal peptide.The identity of nucleotide sequence and amino acid sequence of this region between PRV Fa strain and Rice strain was 97.5% and 94.8%,respectively.