尿毒症患者血清高sTNFR水平及其对TNF活性的抑制

High sTNFR levels in sera of patients with uremia and their inhibition on TNF activity

目的 探讨尿毒症患者的异常免疫状态 ,明确尿毒症患者血清免疫抑制的本质。方法 用双抗体夹心酶联免疫吸附法 (ELISA)检测 30例长期常规每周行血液透析尿毒症患者的血清TNF、sTNFRⅠ、sTNFRⅡ水平 ,并同时观察该血清在体外对TNF细胞活性的影响。结果 尿毒症患者血清中sTNFRI、sTNFRII水平较正常组显著增高 ,而血中TNF水平低于检测低限。尿毒症患者血清具有抑制TNF细胞毒活性作用。结论 尿毒症患者血清高sTNFR水平及其对TNF活性的抑制作用是导致尿毒症患者免疫功能低下的原因之一 。

Objective To investigate the abnormal immune status in patients with uremia.Methods We determined the serum levels of TNF, sTNFR Ⅰ, sTNFR Ⅱ in 30 chronic hemodialysis patients by ELISA. The inhibition of the patients' sera on TNF activity was also observed. Results The serum levels of sTNFRI, sTNFRII were significantly higher in patients with uremia than that in normal controls and the TNF concentrations were below the detectable limit. The serum from patients with uremia had obviously inhibition effect on TNF activity. Conclusion The high levels of sTNFR contribute to the abnormal immune status in patients with uremia and sTNFRs may have some important biological effects in vivo.