紫杉醇脂质体细胞毒作用和抗瘤作用的研究

Study on Cytotoxic Effect and Antitumor Effect of Paclitaxel Liposome

为研究紫杉醇脂质体的体外细胞毒作用及体内抗瘤作用。进行噻唑蓝 (MTT)体外试验法和腹腔给药体内抗瘤试验。发现 :紫杉醇脂质体对体外培养的人卵巢癌细胞 COC1 在浓度为 2 2 .5 μg/ m l、 45 μg/ m l和 90 μg/ m l时 ,其抑制率分别为 78.48%、 84.90 %和 93.5 3%。体内腹腔给予紫杉醇脂质体 5 mg/ (kg· d) ,10 m g/ (kg· d) 8d,对小鼠艾氏癌实体型的抑瘤率分别为 32 .47%和 5 4.6 3% ,二组有显著性差异 (P<0 .0 1) ;小鼠体重变化率分别为 2 2 .11%和 2 1.16 % ,二组间无显著性差异 (P>0 .0 5 )。结果表明 :紫杉醇脂质体在体外显示细胞毒作用 ;

The cytotoxic effect and antitumor effect of paclitaxel liposome were studied by using MTT assay method in vitro and in vivo antitumor test. The inhibitory rate of paclitaxel liposome on human ovarian cancer cell COC 1 was 78 48 %, 84 90 %, 93 53 % respectively at the concentrations of 22 5 μg/ml, 45 μg/ml, 90 μg/ml respectively. Under the doses of 5 mg/(kg·d) and 10 mg/(kg·d) for 8 days, the inhibitory rate of paclitaxel liposome against xenograft of solid Ehrlich tumor in mice was 32 47 % and 54 63 % respectively ( P <0 01). The weight change rate of the mice was 22 11 % and 21 16 % respectively. It was suggested that paclitaxel liposome possessed in vitro and in vivo antitumor activities. The toxicity of liposomal paclitaxel was lower than that of free paclitaxel.