用酶联免疫吸附法测定鱼类体内金属硫蛋白

Study for quantitative determination of Metallothionein with enzyme linked immunosorbent assay(ELISA)

用酶联免疫吸附法测定鱼体内 (肝 ,肾 ) MT。鱼组织经匀浆、加热、离心、将上清液以包被液稀释后 ,与一抗、二抗反应 ,求得 MT含量。与镉 /血红蛋白饱和法比较 ,本法显示了特异、简便、及灵敏的特点。同时 ,用酶联免疫吸附法测定了取自武汉东湖不同水域的鱼类、不同种类的鱼类的组织脏器的 MT的含量 。

Determination of metallothionein of fish tissues(liver and kidney) has been established. Fish tissues were homogenized, heated and centrifuged. The supernatent was used to react with polyclonal\|antibody. Comparing with the cadmium/hemoglobin saturation method, this method was shown distinctive, credible and sensitive.