跨膜糖蛋白和肺耐药相关蛋白在人脑胶质瘤中的表达

Expression of P-glycoprotein and Lung Resistance related Protein in Human Glioma

目的 :探讨人脑胶质瘤组织中跨膜糖蛋白 (P gp)和肺耐药相关蛋白 (LRP)的表达及其与胶质瘤临床病理因素及多药耐药 (MDR)之间的关系。方法 :应用免疫荧光标记的单克隆抗体 ,通过流式细胞术方法 ,检测 30例新鲜人脑胶质瘤中P gp和LRP表达情况。结果 :P gp和LRP在人脑胶质瘤中有过度表达。二者表达程度与胶质瘤恶性程度有关 ,在低度与中、高度恶性胶质瘤之间P gp和LRP表达阳性率差异均有显著性 (P <0 0 5 ) ;P gp和LRP在人脑胶质瘤中有共同表达。结论 :P gp和LRP可作为人脑胶质瘤的化疗敏感性及预后的判断指标 ,二者联合检测对胶质瘤患者预后判断有更大价值。

Objective To explore the interrelationship of P glycoprotein (P gp) and lung resistance related protein (LRP) with pathological type,malignant grading,mutidrug resistance of human glioma.Methods We used anti P gp FITC and anti LRP FITC,by flow cytometric immunology (FCMI) method to detect the P gp and LRP expression in 30 human glioma tissues.Results There was overexpression of P gp and LRP in the human glioma tissues.The rates of positive expression of P gp and LRP has relation with malignant grading of glioma.Significant difference could be seen between low grade and moderate,high grade ( P <0 05).P gp and LRP sometimes had co overexpression in glioma tissues.Conclusions P gp and LRP may become important indices for judging the prognosis and response to chemotherapy of human glioma.Combined detection of P gp and LRP plays a important role in judging the prognosis of human glioma.