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腹膜透析时白细胞介素-8与溶质转运 被引量:1

Study On Correlation Between Interleukin-8 of Solute Transperitoneal Transport Of Peritoneal Dialysis
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摘要 目的 研究IL - 8水平与蛋白质转运之间的关系 ,了解腹膜透析溶质转运机制。方法 将 12只新西兰大白兔随机分为实验组和对照组。实验组和对照组腹腔内分别注入 2 5 %葡萄糖透析液 (40ml/Kg) ,同时实验组腹腔内注入 3× 10 9CFU金黄色葡萄球菌悬液 1ml,而对照组腹腔内注入 1ml生理盐水作为对照。分别测定各时间点透析液和血肌酐、葡萄糖、总蛋白、白蛋白 ,同时测定透析液白细胞介素 - 8的浓度 ,分别计算肌酐、总蛋白、白蛋白D/P值和葡萄糖D/Do值。结果 实验组血肌酐、总蛋白和白蛋白的D/P明显升高及葡萄糖D/Do明显降低 ,与对照组比较均有显著性差异 (P <0 0 5 )。实验组各时间点透析液内白细胞数及中性粒细胞数和IL -8水平升高 ,与对照组比较 ,有显著性差异 (P <0 0 5 )。各时间点透析液内IL - 8水平与总蛋白D/P值明显相关(P <0 0 5 ) ,6 0min以前各时间点透析液内IL - 8水平与白蛋白D/P值无相关性 (P >0 0 5 ) ,但 6 0min以后各时间点则有显著相关性 (P <0 0 1)。结论 腹腔内注射 3× 10 9CFU金黄色葡萄球菌标准菌株可成功制成兔腹膜透析腹膜炎动物模型。IL - 8可影响大小分子蛋白质转运 ,但对大分子蛋白质转运的影响较晚。 Objective In order to understand the mechanism of solute transperitoneal transport, we studied the relationship between IL-8 level and protein transperitoneal transport. Method 12 New Zealand White rabbits were randomly divided into experimental and control groups. 2 5% glucose peritoneal dialysis solution (40ml/kg)contained 3×10 9 CFU staphylococcus aureus 1ml was injected into the abdominal cavity of experimental NZW rabbits and 2 5% glucose peritoneal dialysis solution (40ml/kg)contained 0.9%NaCl solution 1ml was injected into the abdominal cavity of controlling NZW rabbits. The plasma and effluent concentrations of blood creatinine ,glucose,total proteins and albumine were determined respectively, the D/P or D/Do values of creatinine , total proteins ,albumine or glucose were calculated respectively. Results The D/P ratios of creatinine, total proteins and albumin were increased significantly, while the D/Do of glucose was decreased in experimental group on different dwelling time points ,there was a significantly difference,as compared with the control group (P<0 05).Compared with the control group,Il-8 level, WBC number markedly increased (P<0 05).Effluent IL-8 level at different points was related to the D/P ratios of the total proteins (P<0 05).There was no significantly relationship between effluent IL-8 level at different points before 60 mins and the D/P ratios of albumin(P>0 05),while there was significantly correlation after 60 mins(P<0 05).Conclusions Intraperitoneal administration of 3×109CFU staphylococcus aureus standard stains successfully created the rabbit peritoneal dialysis-related peritonitis animal model.IL-8 may early influence the transperitoneal transport of both the large and small molecular proteins,but the effect on the transperitoneal transport of large molecular proteins can appear after dwelling 60 mins.
出处 《中国医师杂志》 CAS 2001年第8期572-575,共4页 Journal of Chinese Physician
关键词 腹膜透析 腹膜炎 动物模型 白细胞介素-8 Peritoneal dialysis Rabbit Peritonitis Animal,model interleukin-8
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