α-生育酚和维生素C抗晶状体氧化损伤的实验研究

The experimental study on the prevention of cataract with α-tocopherol and vitamin C

目的 :观察体外补充α -生育酚和维生素C后氧化损伤晶状体的形态学及抗氧化系统的变化 ,研究α -生育酚和维生素C对晶状体氧化损伤的保护作用。方法 :将正常兔晶状体分为正常培养组 (对照组 ) ,氧化损伤组 (Feton模型组 )和α -生育酚组、维生素C组 ,体外培养 2 4h后观察各组晶状体的混浊情况 ,检测各组晶状体的总抗氧化能力 (T -AOC)水平 ,可溶性蛋白 (slo -Prot)含量 ,抗氧化酶系统中的超氧化物歧化酶(SOD) ,过氧化氢酶 (CAT)及谷胱甘肽过氧化物酶 (GSH -px)活性以及脂质过氧化反应终产物丙二醛 (MDA)的含量。结果 :氧化损伤组晶状体全部混浊 ,对照组、α -生育酚组和维生素C组分别为 8%、2 5 %和 17% ;和对照组相比 ,氧化损伤组T -AOC水平、sol -Prot含量、SOD、CAT和GSH -px活性明显降低 ,MDA含量明显升高 ,α -生育酚组和维生素C组上述指标均有不同程度改善。结论 :α -生育酚和维生素C可提高氧化损伤晶状体的抗氧化能力 ,降低脂质过氧化物水平 ,减少白内障的发生。

Objective:To evaluate the effect of α-tocopherol and vitamin C on protecting lens against oxidative stress.Method:Rabbit's lenses cultured in vitro were divided into 4 groups:control group,Feton model(oxidative stress group),groups treated with 0.01mol/L α-tocopherol and with 0.1mol/L vitamin C.After 24 hours incubation,the transparency of all lenses were examined.The levels of T-AOC,SOD,CAT,GSH-px,sol-Prot and MDA were tested.Results:Lower lenses exposed to α-tocopherol and vitamin C developed lens opacity compared with Feton model.MDA level decreased,while T-AOC,sol-Prot levels,SOD,CAT and GSH-px activities increased significantly in α-tocopherol and vitamin C groups compared with Feton model.Conclusion:α-tocopherol and vitamin C can protect lens against oxidative stress and prevent cataract formation in vitro.