利用食品工厂活性污泥发酵生产PHB的培养基配方研究(一)

Production of Biodegradable Plastics by Fermentation of Active Sludge from Food Plant (I)

采用L934正交表,设定C、N、P三因素三水平的9种不同配方,对广州四明燕塘乳业公司污水处理站的活性污泥进行生长培养基筛选,根据细胞的总增重率对培养基配方进行了直观分析和方差分析,并绘制出细胞的生长曲线。结果表明,活性污泥的最佳生长培养基配方为葡萄糖8g·1000mL-1H2O,NH42SO40.75g·1000mL-1H2O,KH2PO40.75g·1000mL-1H2O;经过12h的培养,活性污泥的生长基本达到平衡。

In present article, the media for growth of active sludge in wastewater treatment plant of Guangzhou Siming Yantang Diary Corporation were selected through L9(34) table of orthogonal arrays by designing nine different prescriptions with 3 elements, i.e. C, N, and P, at 3 levels. The growth curves of the cells were drawn according to the variation of the cell weight. The results showed that, the optimum culture medium for growth gained through various analysis consisted of: glucose 8g·L-1, (NH4)2SO4 0.75 g·L-1, KH2PO4 0.75 g·L-1, respectively. It has been found through the growth curves that after 12 hours of culture, the growth of the active sludge generally reached at balance.