补肾活血法对免疫介导型再障小鼠造血调控因子影响的实验研究

Experimental Study on Influence of the Kidney-Tonifying and Blood-Activating Method on Hematopoietic Modulator in Mice with Immune-Guided Aplastic Anemia

建立免疫介导型再障小鼠模型 ,胃饲补肾活血方药 ,观察造血调控因子IL 2、IL 10、IFNγ、TNFα分泌水平的变化。结果 :免疫介导型再障小鼠造血负调控因子IL 2、IFNγ、TNFα显著高于正常对照组 (P <0 0 5 ) ,IL 10低于正常对照组 ,(P <0 0 5 )。补肾活血中药组IL 2、IFNγ、TNFα显著低于模型组 (P <0 0 5 ) ,IL 10显著高于模型组 (P <0 0 5 ) ,说明补肾活血方药可通过抑制造血负调控因子的分泌 ,减轻免疫因素对机体的损害 。

In order to explore the influence of the compound formula to tonify the kidney and activate blood on hematopoietic function in the patients with aplastic anemia,mice models with immuneguided aplastic anemia were set up and fed with the drugs to tonify the kidney and activate blood,to observe changes in secretion level of hematopoietic modulators IL 2,IL 10,IFNγ and TNFγ.The results showed that IL 2,IL 10,IFNγ and TNFγ were remarkable higher in the model mice(A)than in the control group(B)(P<0 05) and IF 10 was lower than the Group B (P<0 05).IL 2,IFNγ and TNFγ were obviously lower in Chinese medicine group(C)than in the Group A (P<0 05)and IL 10 was remarkably higher than Group B(P<0 05).The findings indicate that the formulas and frugs to tonify the kidney and activate blood can reduce the damage of immune factor on the organism and restore the hematopoietic function of the organism by inhibiting excretion of the hematopoietic modulators.