白三烯C_4对人气道结构细胞内皮素1基因表达及分泌水平的影响

Influence of Leukotriene C_4 on Secretion and Expression of Endothelin-1 Gene in Human Airway Structural Cells

目的 :探讨白三烯C4 (LTC4 )对体外培养人气道上皮细胞和平滑肌细胞内皮素 1(ET 1)基因表达及分泌水平的影响。方法 :在离体培养人气道上皮细胞株 (16 HBE)及气道平滑肌细胞 (ASMC)培养液中加入不同浓度LTC4 ,采用酶联免疫吸附 (ELISA)法测定培养液ET 1水平 ,用逆转录多聚酶链反应 (RT PCR)方法检测ET 1mR NA表达水平。结果 :LTC4 能使离体培养的 16 HBE及ASMC两种细胞ET 1mRNA的表达水平显著上调 ,并刺激ET 1释放 ,当LTC4 浓度≥ 10 -8mol/L时 ,两种细胞ET 1分泌水平均显著高于对照组。结论 :LTC4 能上调 16 HBE及ASMC两种细胞ET 1mRNA的表达并刺激ET 1释放 ,这可能是白三烯致哮喘气道炎症及重塑的重要机制之一。

Objective:To investigate the effects of leukotriene C 4(TC 4) on endothelin 1(ET 1) mRNA expression and ET 1 production in human airway epithelial cell strain (16 HBE)and airway smooth muscle cells (ASMC).Methods:16 HBE or ASMC cells were incubated with different concentrations of LTC 4,ET 1 mRNA expression and ET 1 release from cultured cells were determined by RT PCR and ELISA.Results:ET 1 mRNA expression and ET 1 levels were significantly higher in both 16 HBE and ASMC incubated with LTC 4(≥10 -8 M) compared to those in controls.Conclutions:LTC 4 induces over expression of ET 1 mRNA and ET 1 release in 16 HBE and SMCS,which may be one of the important mechanism of LTs in airway inflammation and reconstraction in asthma.