应用PCR-SSP法分析中国人血小板抗原基因型频率(英文)

Analysis of Human Platelet Antigen Genotypic Frequencies in Chinese Population by PCR Amplification with Sequence Specific Primers

本研究建立了在同一PCR反应条件下同时检测人血小板抗原 (humanplateletantigen ,HPA)系统HPA 1到HPA 5的PCR SSP检测法。应用该方法分析了 110例健康献血员的HPA 1到HPA 5的基因型 ,并以此为依据推算了中国人HPA 1到HPA 5各亚型的基因频率。结果表明HPA 1a和HPA 1b的基因频率分别为 0 .91和 0 .0 9,HPA 2a和HPA 2的基因频率分别为 0 .86和 0 .14,HPA 3a和HPA 3b的基因频率分别为 0 .60和 0 .4 0 ,HPA 4a和HPA 4b的基因频率分别为 0 .92和 0 .0 8,HPA 5a和HPA 5b的基因频率分别为 0 .85和 0 .15。结论 :基因组DNA的血小板抗原PCR SSP分型法切实可行 。

In present study, a method for genotyping for human platelet antigen(HPA) systems by means of the polymerase chain reaction amplification with sequence-specific primers (PCR-SSP) technique was developed and employed to determine the human platelet antigen frequencies in the Chinese population. Primers sets were designed to allow PCR amplification for five systems using the same assay conditions. Platelets from 110 random Chinese blood donors were typed for human platelet alloantigens HPA-1 to -5 by the method. The results showed that the HPA genotypic frequencies observed in the 110 donors were 0.91 and 0.09 for HPA-1a and HPA-1b, 0.86 and 0.14 for HPA-2a and HPA-2b, 0.60 and 0.40 for HPA-3a and HPA-3b, 0.92 and 0.08 for HPA-4a and HPA-4b, and 0.85 and 0.15 for HPA-5a and HPA-5b, respectively. In conclusion the method is feasible and practical and may be available to typing for HPA in the clinical laboratories.