吲达帕胺、硫氮酮对缺血/再灌注心肌的保护作用

Experimental study on protective effects of indapamide and diltiazem against myocardial ischemia reperfusional injury

目的 采用离体大鼠心脏灌注模型 ,探讨吲达帕胺 (Indapamide)、硫氮酮 (Diltiazem)在心肌缺血 /再灌注过程中对心肌的保护作用。方法 SD大鼠 45只 ,随机分为吲达帕胺组、硫氮酮组、对照组 (灌注液中不含药物 )。比较三组动物在心肌缺血 30min及再灌注 30min后心电图、心肌酶 (CK、LDH) ,同时监测心功能指标的变化。结果 ①对照组在心肌缺血 /再灌注后CK、LDH含量较缺血前明显升高 (P<0 0 5 )。吲达帕胺组、硫氮酮组在心肌缺血 /再灌注后心肌CK、LDH含量较对照组明显降低 (P <0 0 1)。②吲达帕胺组、硫氮酮组于再灌注 15、30min两时点 ,冠脉流量、心肌收缩力恢复百分比显著高于对照组 (P <0 0 5 )。结论 吲达帕胺、硫氮酮对缺血

Objective To study the protective effects of indapamide and diltiazem myocardial on ischemia reperfusion injury in isolated rat hearts. Using a langendorff apparatus. Methods 45 healthing Sprague-Dawley rats were randomly divided into 3 groups: indapamide group, diltiazem group and control group. Assessments of electrocardiogram, myocardial enzyme, hemodynamics at 30 minutes after ischemic cardiac arrest and 30 minutes after reperfusion.Result ①At 30 minutes after ischemic cardiac arrest and 30 minutes after reperfusion. In group control, CK and LDH were much higher than before ischemic cardiac arrest ( P<0 05 ). After 30 minutes of ischemia and 30 minutes of reperfusion, CK and LDH in indapamide group and diltiazem group were slightly lower than those in control group. ② After reperfusing for 15 minutes and 30 minutes, in indapamide group and diltiazem group , the recovery percentagt of HR, contractie power and coronary flow were remarkably higher than that of control group( P<0 05 ).Conclusion Indapamide and diltiazem have protective effects against myocardial ischemia-reperfusional injury.