大鼠脊髓损伤后血脊髓屏障通透性变化的观察

Study of blood-spinal cord barrier disruption after spinal cord injury in rats

目的 :探讨脊髓损伤后血脊髓屏障通透性变化的机理。方法 :采用美国纽约大学 (NewYorkUniversity ,NYU)脊髓损伤模型 ,选用体重 30 0～ 35 0克雄性成年Wistar大鼠 35只 ,随机分为对照组 5只 ;脊髓损伤组 30只 ,分为伤后 4、6、12、2 4、48、72h 6组 ,每组 5只 ,应用NYU脊髓损伤模型 ,采用免疫组织化学方法 ,观察脊髓损伤后不同时间免疫球蛋白G(immunglobularproteinG ,IgG)、补体 3的C片断 (cfragmentofcomplement3,C3c)的表达变化。结果 :脊髓损伤后C3c、IgG渗入了脊髓损伤区域、损伤区域周边及血管 ,脊髓损伤不同时间段这些区域的免疫标记不同。结论 :脊髓损伤后C3c、IgG参与了血脊髓屏障的破坏 。

Objective:Investigate the mechanism of blood spinal cord barrier disruption after spinal cord injury.Method:Thirty five male adult Wistar rats(300～350g) were randomly assigned to this study,there were one control group and six spinal cord injury groups(acording to the time of post injury,4h,6h,12h,24h,48h and 72h).Each group contained 5 rats and New York University (NYU) Spinal Cord Injury Model was utilized to create the spinal cord injury.The immunoexpressior changes of immunglobularprotein G(IgG),c fragment of complement3 (C3c) in different time after spinal cord injury were evaluated utilizing immunohistochemistry method.Result:After spinal cord injury, there was a marked chang in the immunoexpressior of IgG and C3c in the impact site, near the impact site and spinal cord microvascular.Conclusion:After spinal cord injury,IgG and C3c may be important factors of barrier disruption and related to neuron secondary injury.