曲酒发酵池中兼性自养型链霉菌的分离与特征研究

Study on Isolation and Characteristics of Facultatively Autotrophic Streptomyces in Fermentation Pit of Daqu Chinese Liquor

论述了浓香型曲酒发酵池窖泥中兼性自养型链霉菌 (DS - 0 2 1)的分离、鉴定及其特征方面的研究情况。采用无机基础培养基分离的菌株为能氧化硫及硫化物的兼性自养型链霉菌 ,它属于放线菌目链霉菌科链霉菌属的金色类群 ,具有利用硫及硫化物自养生活和利用有机物进行异养的能力。特征研究表明 :DS - 0 2 1最适的复合液态培养基为MCP ;在乙醇体积分数为 5 %条件下生长良好 ;液态培养的最适 pH在 4～ 5之间。当DS - 0 2 1菌液添加量在1 8%或 2 0

A facultatively autotrophic streptomyces(DS 021)was isolated from the fermentation pit of Daqu Chinese liquor and its characteristics were researched This strain could utilize autotrophically sulphur compound for growth and metabolism The optimum compound medium chose for expanding culture was modified corn powder(MCP) It also showed that DS 021 had the good growth when the medium contained 5% of ethyl alcohol and the pH value of the medium was between 4～5 Optimum actions of promoting growth were occured to Clostridium caproicum and ester forming yeast by adding 1 8%,2 0% respectively of fermented broth of DS 021 to the media of above two microorganisms