黑龙江立克次体蜱传斑点热的病原学研究

Study On Aetiology of Rickettsia Heilongianggii Tick Borne Spotted Fever

目的 对我国东北地区蜱传斑点热进行病原学和流行病学研究。方法 建立并应用分子立克次体学方法 ,包括单克隆抗体、SDS -PAGE、免疫印迹、DNA酶切图谱、PCR/RFLP、PCR/SSCP及DNA序列分析等。结果 (1)在种的水平上研究了黑龙江分离株 (代表株HLJ - 5 4、36) ,从分类学上确定其属立克次体属、斑点热群的一个新种 ,命名黑龙江立克次体(R heilongiangii) ;(2 )发现其现症患者 ,并从病人血液分离到一株与黑龙江立克次体相同性质的立克次体株 ,证实了其对人的致病性。

Objective To study the aetiology and epidemiology of tick borne spotted fever in northeastern area.Method Monoclonal antibodies,immunohlot-ting,endonuclease restiction analysis of DNA,PCR/RFLP.PCR/SSCP and DNA sequencing were used.Results (1) The results demonstrated that R.heilongjiangii was different from other international reference strains of SFGR and was proposed to be a new species of SFGR.(2)R.heilongjiangii and H-5 strain from a Patient′s blood specimen were same species,which has ability to cause disease in human.Conclusion The investigation with epidemiology demonstrated that Dongning and Luobei of Heilongjiang province and Hunchun area of Jilin province are the nature foci of R heilongjiangii tick borne spotted fever.