精神分裂症患者中单胺氧化酶A和B多态性位点的连锁不平衡研究

Study on Linkage Disequilibrium Between Polymorphic Loci for Monoamine Oxidases A and B in Schizophrenia

目的 为研究单胺氧化酶与精神分裂症的关系。方法 采用聚合酶链式反应 ,对 140例无血缘关系的高加索男性精神分裂症患者和 91例对照者的两个X连锁的微卫星序列即单胺氧化酶A位点中的 (AC)n重复序列和单胺氧化酶B位点的 (TG)n等位基因进行了研究。结果 基因分布频率在病人组和对照组中未发现差异。但 (AC) 18(TG) 2 3 单倍体增多与精神分裂症有关 ,相对危险度是 4 0 5 (95 %CI 1 15～ 14 2 6) ,精确概率P =0 0 11,连锁不平衡系数在精神分裂症组是 0 0 19;在对照组是 - 0 0 46。

Objective To search for the association between monoamin eoxidases and schizdophrenia, two X-linked microsatellites,(AC)n repeats at the monoamine oxidase(MAO) A and locus and (TG)n repeats at the MAO B locus.Mothods PCR was used to study (AC)n repeats and (TG)n repeats in 140 unrelated Caucasian male patients with schizophrenia and 91 unrelated Caucasiancontrols.Results Allelic frequencies of either (AC)n repeats or (TG)n repeats were not found to be significantly different between patients and controls. However, a significant excess of the (AC) 18 /(TG) 23 haplotype with a relative risk of 4 05(95%CI 1 15～14 26)was observed in patients with schizophrenia(Fisher's p=0 011). The coefficient of linkage disequilibrium for the (AC) 18 /(TG) 23 haplotype was 0 019 in schizophrenic patients and -0 046 in controled subjects, respectively.Conclusion The present findings suggested that linkage disequilibrium between polymorphic loci for human MAO-A and MAO-B might be associated with schizophrenia.