Cu、Zn、Pb、Cd及其混合重金属离子对鲫鱼(Carassiusauratus)DNA甲基化水平的影响

Influence of Cu, Zn, Pb, Cd and their heavy metalion mixture on the DNA methylation level of the fish (Carassius auratus).

在重金属离子的暴露作用下,鲫鱼DNA总甲基化水平发生变化.结果表明,Cu、Zn、Pb、Cd及其混合重金属离子极大地提高了鲫鱼肝脏DNA的总甲基化水平;Cu、Zn两种生物元素对DNA总甲基化水平的改变要小于Pb、Cd两种非生物元素;随着混合重金属离子浓度的增加,鲫鱼肝脏DNA的总甲基化水平也有所增高;混合重金属离子对不同组织DNA总甲基化水平的影响不同,肝脏>鱼鳃>肾脏.重金属离子作用下的鲫鱼组织DNA总甲基化水平改变是其产生基因毒性作用的另一种机制.

Under exposing to heavy metal ions, there happens change of the total methylation level of the fish DNA. Results show that the copper, zinc, lead, cadmium and their heavy metal ion mixture increase significantly the total level of the fish liver DNA methylation; two bio-elements (copper and zinc) change the total level less than two non-bio-elements(lead and cadmium), the level of DNA methylation in the fish liver increases with enhancing concentration of the heavy metal ion mixture; and the influence of the mixture to the total DNA methylation in different tissues also differs with liver >gill>kidney. The change of the total DNA methylation level in there tissues under the action of heavy metal ions is another mechanism of the genotoxicity of heavy metal for the fish.