几种生长因子在人表皮细胞无血清培养中的作用

The Roles of Several of Growth Factors in Serum free Culture of Human Epidermal Cells in Vitro

采用无血清培养系统，以３Ｈ－ＴｄＲ掺入法测定表皮生长因子（ＥＧＦ）、牛垂体提取物（ＢＰＥ）和乙醇胺（Ｅｔｈａ）对人表皮细胞ＤＮＡ合成的影响。结果显示，ＥＧＦ和ＢＰＥ在本实验所建立的无血清培养基中对培养表皮细胞ＤＮＡ合成有明显的促进作用（Ｐ＜０．０１）；Ｅｔｈａ对培养表皮细胞ＤＮＡ合成也有促进作用（Ｐ＜０．０５），ＥＧＦ和Ｅｔｈａ在本培养体系中具有协同作用（Ｐ＜０．０５）。

This study was established in serum free condition.The effects of epidermal growth factor (EGF),bovine pituitary extract (BPE), and ethanolamine (Etha) on the DNA synthesis of human epidermal cells were determined by thymidine incorporation.EGF and BPE were shown to promote the DNA synthesis of cultured epidermal cells significantly ( P <0 01).Etha exerted a positive influence on it ,too (P <0.05).A mixture of EGF and Etha yielded better growth than either alone ( P <0.05).The possible mechanism and applied perspective of the growth factors used for burn were discussed.