血清丙肝病毒RNA二种提取方法的比较

Comparison between the Two Methods of Extracting HCVRNA from Sera

２４例抗－ＨＣＶ阳性的血清标本，同时以硫氰胍半字符热酚变性方法和蛋白酶ＫＩ肖化法裂解提取丙肝病毒ＲＮＡ（ＨＣＶＲＮＡ），并应用反转录半字符多聚酶链反应（ＲＴ－ＰＣＲ）和套式多聚酶链反应（Ｎｅｓｔ－ＰＣＲ）检测ＨＣＶＲＮＡ。结果表明，硫氰胍半字符热酚法明显优于蛋白酶Ｋ消化法。前者处理标本的阳性检出率为５８．３％，后者处理标本的阳性检出率为３３．３％（Ｐ＜０．０５）；前者操作步骤简便，在试剂的保存等方面优于后者。

Serum virus RNA of hepatitis C(HCV RNA) was extracted from 34 anti HCV positive patient using proteinase K digestion technique and guanidinium thiocyanate hot phenol technique.HCV RNA were detected with reverse transcription (RT) PCR and Nest PCR.The result exhibited that guanidium thiocyanate hot phenol technique is far better than proteinase K diges tiont echnique .the detectable rate for positive HCV RNA was 58.3% while with the latter technique it was 33.3%( P <0 05).Furthermore,the operative procedure of the former technique was simpler and easier,and the sforage splitting reagents of the former was better than those of the latter technique .It is suggestible to develope the guanidinium thiocyanate hot phenol technique to extract HCVRNA clinically.