非甾醇类抗炎药物对晶状体抗氧化酶失活的保护作用

Protection of nonsteroidal anti inflammatory drugs against inactivation of antioxidant enzymes

目的 研究非甾醇类抗炎药物对抗氧化酶失活的作用 .方法 以超氧化物歧化酶 ( SOD)和过氧化氢酶 ( CAT)分别与糖加阿斯匹林、布洛芬和扑热息痛孵育 ,于相应的孵育时间采用分光光度计测定酶的活性 .结果 糖与酶的孵育导致时间依赖性的 SOD和 CAT失活 .果糖表现出比 6-磷酸葡萄糖 ( G6P)和葡萄糖更快速的失活效应 .酶孵育 6d后 ,2 0mmol· L- 1阿斯匹林可部分保护 5 0 mm ol· L- 1果糖和 G6P诱导 SOD的失活 ,5 mm ol· L- 1 G6P和 10 mmol· L- 1 葡萄糖诱导 CAT的失活 ,未观察到保护 5 mmol· L- 1 果糖诱导CAT的失活 .而布洛芬有限地保护糖基化诱导 CAT的失活 ,对 SOD的失活无保护作用 .扑热息痛对两者均无保护作用 .结论 阿斯匹林和布洛芬可部分保护糖基化诱导 CAT和 SOD的失活 .这可能在阿斯匹林类药物抑制白内障发病的作用机制中起重要作用 .

AIM To evaluate the protection of nonsteroidal anti inflammatory drugs against inactivation of antioxidant enzymes. METHODS The superoxide dimutase (SOD) and catalase (CAT) were incubated with sugar or with aspirin,ibuprofen and paracetamol, respectively. The enzyme activities were assayed by the spectrophotometer and expressed relative to the control activity at each respective incubation time. RESULTS Fructose, glucose 6 phosphate (G6P) and glucose inactivated the SOD and CAT in a time dependant manner. Fructose displayed a more rapid inactivation effect than G6P and glucose. After incubation 6 days, 20 mmol·L -1 aspirin partially protected SOD against inactivation by 50 mmol·L -1 fructose and G6P and also protected CAT against inactivation by 5 mmol·L -1 G6P and 10 mmol·L -1 glucose. No protection was observed against inactivation of CAT by fructose. Ibuprofen showed a limited protection CAT against inactivation by fructose, G6P and glucose but not SOD. Paracetamol offered no protection SOD and CAT against glycation induced inactivation. CONCLUSION Aspirin and ibuprofen show partial protection against the glycation induced inactivation of SOD and CAT. This may play an important role in preventing cataract by aspirin like analgesics.