高效液相色谱法测定不同产地羌活中紫花前胡苷的含量

Determination of Content of Nodakenin in Notopterygium incisum Ting from Different Source by HPLC

目的 :测定不同产地羌活中紫花前胡苷含量。方法 :高效液相色谱法。结果 :紫花前胡苷回归方程为Y =- 95 0 9.1+1912 70 9.8X ,r =0 .9998,线性范围 0 .16～ 0 .6 4μg ,平均回收率 99.3% ,RSD 2 .6 4%。 结论 :方法简单、快速、重现性好。

Objective:To develop a method for the determination of nodakenin in N. incisum. Method:A HPLC method was used. Result: Y=-9509.1+1912709.8X,r =0.9998.The calibration was linear in range of 0.16～0.64μg.The average recovery was 99.7%, RSD 1.1%.Conclusion:The method is appropriate for the detemination of nodakenin.