青蒿琥酯对内毒素诱导的一氧化氮合成的抑制作用

Inhibitory Effect of Artemin on Endotoxin-induced Nitric Oxide Synthesis

目的 :探讨青蒿琥酯对内毒素诱导的巨噬细胞一氧化氮 (NO)合成的影响。方法 :①用内毒素 (LPS)或LPS合并γ 干扰素作为巨噬细胞 (RAW 2 6 4.7)的NO合成诱导剂 ,加入不同浓度的青蒿琥酯 ,培养后取上清液 ,用Griess试剂测定NO产生量。②Balb/c小鼠肌肉注射青蒿琥酯 5 0mg·kg-1·d-1× 3d ,收集腹腔巨噬细胞 ,测定LPS对细胞的NO诱生能力。结果 :LPS 1.0 ,0 .2 μg·ml-1或γ 干扰素 10 0u合并LPS 1.0 ,0 .2 ,0 .0 4μg·ml-1作用于RAW 2 6 4.7细胞 ,均可诱导大量NO合成。青蒿琥酯对LPS或LPS合并干扰素诱导的NO合成均有明显的抑制作用 ,其抑制作用具有明显的量效关系。经青蒿琥酯治疗后的小鼠 ,其腹腔巨噬细胞对LPS的反应性降低 ,其受LPS刺激后产生的NO量明显降低。结论 :青蒿琥酯可降低LPS诱导的炎性因子的产生 。

To investigate the effect of Artemin on LPS induced nitric oxide synthesis in macrophages. Method: ① Nitrite oxide (NO) production of RAW 264.7 cells was induced by LPS or LPS in combination with interferon γ(IFNγ) in the presence or absence of Artemin. The amount of NO in the supernatant of RAW 264.7 cells was detected with Griess reagent. ② Balb/c mice were injected with Artemin (im) 50 mg·kg -1 ·d -1 ×3d, and intraperitoneal macrophages were collected to detect the LPS induced NO production. Result: LPS 1.0, 0.2 μg·ml -1 or IFN γ100u+LPS 1.0,0.2, 0.04 μg·ml -1 could induced a large amount of NO synthesis of RAW 264.7 cells. Artemin showed a significant inhibitory effect on LPS or IFN γ+LPS induced NO production in a dose dependent manner. After treatment with Artemin, the response of Balb/c mice to LPS was reduced, which was showed by a decrease in NO production of intraperitoneal macrophages induced by LPS. Conclusion: Artemin could reduce LPS induced production of inflammatory factors resulting in the inhibition of inflammatory effects. [