穴注法对大鼠慢性萎缩性胃炎胃粘液屏障的影响

Effect of Point Injection Method on Gastric Mucosa Barrier in Rats with Chronic Atrophic Gastritis

目的 观察穴位注射对大鼠不同程度慢性萎缩性胃炎 (CAG)时胃粘液屏障的影响。方法 以不同浓度的N 甲基 N 硝基亚硝基胍 (MNNG)复制出不同程度的大鼠CAG模型。在成功造模的基础上 ,以黄芪注射液与当归注射液等分混合穴注足三里对其进行治疗 ,观察其对CAG病变情况及胃粘膜氨基己糖与磷脂值的影响。结果 胃粘膜损伤指数、萎缩、肠化、异型增生或两者合见者随着造模浓度的增加而增加 ,穴注组可明显改善胃粘膜损伤指数及治疗相关病变 (P均<0 .0 1) ;随着造模浓度的增加 ,胃粘膜氨基己糖及磷脂值显著下降 ,而穴注组可明显提高这两项指标 ((P均 <0 .0 1) ;胃粘膜损伤指数与氨基己糖及磷脂值呈负相关 (P均 <0 .0 1)。结论 随着CAG程度的加重 ,胃粘膜氨基己糖及磷脂值明显下降 ,胃粘液屏障功能受损。穴注法可明显提高胃粘膜氨基己糖及磷脂值 ,通过加固胃粘液屏障 。

OBJECTIVE To observe the effect of the point injection method on the gastric mucosa barrier in rats with chronic atrophic gastritis. METHOD Rat models with varying degrees of chronic atrophic gastritis were made using MNNG in different concentrations. Equal amounts of Huangqi Injection and Danggui Injection were mixed and injected into the point Zusanli to observe the effect of the injection on the pathological change, hexosamine of the gastric mucosa and the value of phospholipid. RESULT The indexes of the gastric mucosa damage, atrophy, metatypical hyperplasia, etc. increased with the increase of the concentration in model making. In the point injection group, marked improvement was detected in gastric mucosa damage and other related pathological changes ( P <0.01). With the increase of the concentration in model making, hexosamine and phospholipid of the gastric mucosa assumed negative correlation ( P <0.01). CONCLUSION With the increase of the severity of chronic atrophic gastritis, the values of hexosamine and phospholipid drop significantly and the function of the gastric mucosa barrier is impaired. Point injection can markedly increase the values of hexosamine and phospholipid and prevent the occurrence of chronic atrophic gastritis by reinforcing the gastric mucosa barrier.