利用鸡蛋抗体防止变形链球菌的再生

Application of egg-yolk-antibody to prevent recolonization of Streptococcus mutans

目的 :研究局部运用含有特异性鸡蛋抗体的水溶性蛋白溶液对口腔中变形链球菌再生的影响。方法 :用灭活的变形链球菌整个细胞做为抗原免疫母鸡 ,然后从鸡蛋中分离出含有特异性鸡蛋抗体的水溶性蛋白制成漱口水 ,进行人的体内实验。结果 :用含特异性抗体浓度为 10 0 mg/ L 的水溶性蛋白溶液漱口 ,在 10 0 d内可以完全抑制志愿者口腔中变形链球菌的再生。结论 :利用含抗变异链球菌鸡蛋抗体的水溶性蛋白可以有效防止变形链球菌在口腔中的再生。作为预防龋齿的方法 ,方法简便、安全有效。

Objective:To investigate the effect of local application of the water soluble protein fraction,containing specific egg yolk antibody,on the recolonization of Streptococcus mutans.Methods:Whole cells of inactivated streptococcus mutans were used as antigen to immunize bens,then water soluble protein fraction(WSF) was extracted from the eggs. Mouth wash containing 0.1 mg/ml of WSF was prepared and administered to 8 volunteers.The mouth wash was used once every two days for 2 weeks.Vehicle solution was used in other 6 volunteers as the control.S.mutans in saliva was monitored for 100 days.Results:Before using the mouth washes,S.mutans level in saliva of the volunteers was 36.4%.S.mutans was removed by hibitane,it kept less than 3% in 100 days in the tested individuals,while 23%～37% in the controls.Conclusion:The WSF containing specific egg yolk antibody can effectively prevent the recolonization of streptococcus mutans.