强力霉素对腺样囊性癌细胞系SAcc83增殖及侵袭行为的影响

Effects of doxycycline on the proliferation and invasion of adenoid cystic carcinoma SAcc83 cells

目的 :研究强力霉素对腺样囊性癌细胞增殖、 型胶原酶活性及侵袭行为的影响。方法 :采用 MTT法研究不同浓度的强力霉素对 SAcc83细胞增殖的影响 ,明胶底物 SDS- PAGE凝胶电泳检查 型胶原酶活性 ,单层细胞器官培养进行细胞侵袭研究。结果 :不同浓度的强力霉素抑制了 SAcc83细胞的增殖 ,同时它也抑制了 型胶原酶 (MMP- 2 )活性和肿瘤细胞的侵袭。结论 :强力霉素对 SAcc83细胞的增殖具有抑制作用 ,同时也可抑制肿瘤细胞的侵袭 。

Objective:To study the effects of doxycycline on the proliferation and invasion of adenoid cystic carcinoma cells.Methods:MTT assay was applied to study the effects of doxycycline on proliferation of SAcc83 cells. Activity of type IV collagenase was detected by the method of gelatin incorporated SDS PAGE electrophoresis.Monolayer cell organ culture was carried out to study the invasion of the cells.Results:Doxycycline at 5～50 μg/ml inhibited the proliferation of adenoid cystic carcinoma cell in a dose and time dependant manner. 10 μg/ml of the drug inhibited type Ⅳ collagenase and decreased the invasion of adenoid cystic carcinoma cells by 48%.Conclusion:Doxycycline can inhibit proliferation and invasion of SAcc83 cells.