细胞凋亡过程中核糖体蛋白质S3a水平变化的研究(二)——制备和纯化抗GST/S3a多克隆抗体

The RPS3a level in the process of cellular apoptosis: II. Preparation and purification of polyclonal antibody to GST/S3a

目的:制备和纯化抗GST/S3a多克隆抗体。方法:盐析法粗提抗体,用蛋白A-琼脂糖柱纯化IgG,ELISA法检测多克隆抗体水平。结果:ELISA法测定制备和纯化抗GST/S3a多克隆抗体的活性正常,产生了抗原-抗体反应复合物。结论:制备和纯化的抗体血清可以与GST/S3a融合蛋白相结合,产生抗原-抗体反应复合物,说明具有抗GST/S3a融合蛋白的活性。为下一步实验奠定基础。

Objective: To prepare and purify polyclonal antibody to GST/S3a. Methods: The antibody was extracted by salting method ,the IgG was purified with protein A-agarose column and the polyclonal antibody was detected by ELISA. Results: The acitivity of the polyclonal antibody to GST/S3a was normal which showed the production of producing antigen-antibody response compound. Conclusions: The prepared antibody serum has the activity to the fusion protein GST/S3a, which provides a base for the next step.