高压氧对小鼠aGVHD时细胞因子浓度的影响

Influence of hyperbaric oxygen on cytolcine concentration in mouse a GVHD

目的:观察异基因骨髓移植小鼠,经高压氧作用后,血清中促进aGVHD的细胞因子TNF-α、IL-2及抑制aGVHD的细胞因子IL-4、IL-10浓度及比值的变化,探讨HBO抗aGVHD机理,为HBO的临床应用提供实验依据。方法:复制aGVHD动物模型,经高压氧作用后,用ELISA法检测血清细胞因子浓度并计算比值。结果:经高压氧作用后,血清中细胞因子浓度及比值降低。结论:HBO可明显抑制aGVHD,提高aGVHD小鼠的生存率。

Objective: To observed the effect of HBO (hyperbaric oxygen) on the concentration of serum TNF-α, IL-2, IL-4, IL-10 and IL-2/IL-4, IL-2/IL-10, TNF-α/IL-4, TNF-/IL-10 in mice after allogenic bone marrow transplantation. We probed the mechanism of HBO antagonizing aGVHD and provided experimental data to clinic application of HBO. Method: Duplicated of The model of aGVHD was duplicated and the mice were treated with HBO and serum cytokines determined by ELISA. Result: After HBO, the concentration and ratio of serum cytokines decreased. Conclusion: HBO could inhibit aGVHD remarkably and raise aGVHD mice survival rate.