摘要
目的:研究三甲氧苄嗪(10-6M)对离体心脏低温保存的影响,并探讨其作用机制。方法:以改良的Langendorff离体鼠心灌注模型,观察三甲氧苄嗪加入UW液后(三甲氧苄嗪-10-6M),离体鼠心在4℃保存6小时后血流动力学、冠脉流出液心肌酶漏出量、心肌含水量、心肌ATP、MDA含量、心肌超微结构的变化。结果:UW液加入三甲氧苄嗪组与未加入三甲氧苄嗪组相比,复灌后心功能恢复率明显增高(P<0.01); 心肌含水量(P<0.05)及丙二醛含量较低(P<0.01);心肌酶漏出量明显减少(P<0.01);心肌ATP水平得到很好地维持(P<0.01);心肌超微结构尤其是线粒体结构改变轻,损伤小。结论:10-6M三甲氧苄嗪对低温保存的大鼠心脏具有明显的保护作用。
Objective:To evaluate the effect of 10-6M trimetazidine on rat hearts during cold storage . Methodmodified Langendorff perfused isolated rat heart model , we added trimetazidine to UW solution with its final concentration of 10-6M . After preserved in the UW solution with or without trimetazidine at 4℃ for 6 hours , the following items were observed in the hearts. the changes of hemodynamics , myocardial enzyme leakage in the coronary effluent , myocardial tissue ATP content , MDA content , myocardial water content and the ultrastructural changes of myocardium . Results:Compared myocardium in UW solution , the hearts in UW solution containing 10-6M trimetazidine had higher recovery rate of cardiacfunction and myocardial tissue ATP content (P<0.01) , lower myocardial water content (P<0.05) , myocardial tissue MDAcontent and myocardial enzyme leakage (P<0.01) , slighter ultrastructural change of myocardium, especially the mitochondrialdamage . Conclusions:Trimetazidine(10-6M) could protect the hypothermically preserved rat heart.
出处
《黑龙江医药科学》
2001年第5期8-9,共2页
Heilongjiang Medicine and Pharmacy
