复合微量营养素提高热适应能力的作用及其机理探讨

Effect of multi-micronutrient on heat adaptation and its probable mechanism

复合微量营养素由维生素C、B1 、B2 、B6 、烟酸、叶酸、K、Na、Ca、Mg、Cu、Mn、Fe、Zn等组成。为了探讨其提高热适应能力的作用及其作用机理 ,观察了该营养剂对热暴露果蝇存活率、小鼠热耐力、小鼠高温游泳时间、热暴露大鼠神经内分泌变化、抗氧化功能和对热应激蛋白HSP70合成的影响。结果显示 :(1)复合微量营养素能显著增加热暴露果蝇的存活率 ,延长热暴露小鼠的存活时间和热环境条件下的游泳时间 ;(2 )能明显增加热暴露大鼠肝细胞液糖皮质激素受体结合活性 ,抑制肝组织磷脂酶A2 活性 ;(3)能显著提高热暴露大鼠血清超氧化物歧化酶活力 ,抑制血清丙二醛含量的增加 ;(4)能显著提高热暴露果蝇的热应激蛋白HSP70含量。结果表明 ,复合微量营养素提高热适应能力的作用机理可能与改善神经内分泌调节、提高抗氧化功能和增加热应激蛋白 (HSP70 )合成有关。

A multi micronutrient compound is composed of ascorbic acid, thiamin, riboflavin, vitamin B 6, niacin, folic acid, K, Na, Ca, Mg, Cu, Mn, Fe and Zn. In order to explore the effect of the multi micronutrient on heat adaptation and its probable mechanism, heat exposed Drosophila melanogaster, mice and rats were observed. The results showed that the multi micronutrient could significantly increase the survival rate of heat exposed Drosophila melanogaster and prolong the survival time and swimming time of mice under high temperature. The results indicated that the significant influence of multi micronutrients on heat adaptation could be the result of enhancing the glucocorticoid receptor activity in hepatic cytosol in the heat exposed rats, inhibiting liver phospholipase A2 activity in the heat exposed rats, increasing serum SOD activity and decreasing serum MAD content in the heat exposed rats, as well as increasing level of HSP70 in the heat exposed Drosophila melanogaster. The results suggested that the effect of enhancing heat adaptation might be related to improving neuroendocrine regulation, enhancing anti oxidative function against the damage of free radicals as well as increasing HSP70 content.