花生种子受黄曲霉菌侵染后若干生化成份的变化及其与抗性的关系

Changes of some biochemical substances in peanut seeds under infection of Aspergillus flavus and their role in resistance to seed invasion

花生种子人工接种黄曲霉菌 (AspergillusflavusLink)侵染的生化测试结果表明 ,接种前后花生种子的过氧化物酶 (POX)、多酚氧化酶 (PPO)和苯丙氨酸解氨酶 (PAL)活性以及木质素含量等均发生显著变化。接种前POX活性与侵染率呈极显著负相关 ,接种后POX活性随感染天数增加而增加 ,感病品种比抗病品种POX活性增幅大。接种后 13d抗病品种PPO和PAL活性达峰值 ,而感病品种 36d达峰值。接种后第7d木质素含量与感染率呈显著负相关 (r =- 0 .740 6) 。

The activities of POX, PPO, PAL and content of lignin in peanut varieties with different resistance to Aspergillus flavus invasion were analyzed by artificial inoculation. The results showed that resistance to A.flavus invasion was positively correlated with POX acitivity in uninoculated seeds. The POX activities of resistant and susceptible varieties gradually increased after inoculation. The PPO activity of susceptible varieties increased later than that of resistant varieties, but was higher. The PAL activities of resistant varieties increased rapidly and reached their maximum at the first day, but the susceptible varieties at 4th or 5th day. There was significant negative correlation between PAL activity and infection rate at first day after inoculation. PAL activity might play an important role in resistance to A.flavus invasion in peanut seeds. There was significant negative relation between lignin content and infection rate at 7th day after inoculation.