大鼠休克复苏后肠绒毛与内毒素的定量分析

Quantitative Analysis of the Interstinal Villi and Endotoxin in Rats Following Shock and Resuscitation

目的研究大鼠休克复苏后小肠绒毛和血内毒素含量的改变及两者的相关性。 方法以大鼠经股动脉放血及自体血回输的方法复制创伤性休克及复苏模型。 结果实验组小肠绒毛高度显著低于对照组相应时间点 ,以复苏后 2 4h最低 ,并显著低于休克末 ,复苏 3d有所升高 ,复苏后 7d绒毛高度与休克末已无显著差异 ,但仍低于对照组相应时间点和休克前 ;实验组血内毒素明显高于对照组相应时间点 ,且复苏后血内毒素高于休克末 ,尤以复苏后 2 4h为最高。 结论创伤性休克及复苏后肠粘膜的机械屏障功能降低和血内毒素含量增加 ,且绒毛高度与血内毒素变化呈显著负相关。

Objective To investigate experimentally the changes of intestinal mucosal morphology in correlation with endotoxin during shock and resuscitation after trauma. Methods The experimental model was made in rats, by a 3cm ventral laparotomy, blood letting and reinfusing the autogenous blood through the right femoral artery. Results The intestinal villi in the experiment group following traumatic shock and resuscitation were shorter than those in the control group. The intestinal villi at 24h following resuscitation were the shortest, and were significantly shorter than those at the end of shock. The intestinal villi recovered partially 3d following resuscitation. They didnt show significant difference between at the end of shock and 7d after resuscitation, but were significantly shorter than those of the control group. The endotoxin in the portal vein blood in the experimental group following shock and resuscitation was higher than that in the corresponding control group, and after resuscitation it was higher than at the end of shock; the highest was at 24h after resuscitation. Conclusion These results showed traumatic shock and resuscitation may cause dysfunction of the mechanical barrier of intestinal mucosal and endotoxemia. Significant negative correlation exists between villi and endotoxin in the portal vein.