全反式维甲酸对急淋白血病骨髓基质细胞粘附能力的影响

The Effects of All-trans Retinoic Acid on the Adhesion of Bone Marrow Stromal Cells from Acute Lymphoblastic Leukemia

【目的】探讨全反式维甲酸 (ATRA)对急性淋巴细胞白血病 (ALL)骨髓基质细胞 (BMSC)粘附能力的影响。【方法】将ATRA加进骨髓基质细胞培养体系 ,培养 18h后用流式细胞仪检测 6例ALL骨髓基质细胞表达粘附分子ICAM 1和VCAM 1阳性率 ,用MTT方法检测ALL骨髓基质细胞对正常骨髓造血细胞或淋巴瘤Raji细胞的粘附率。【结果】药理浓度的ATRA(1 0 μmol/L)使ALL骨髓基质细胞ICAM 1表达明显增高 (P <0 0 5 ) ,使ALL骨髓基质细胞对正常骨髓造血细胞或肿瘤细胞的粘附率均明显增高 (P <0 0 5 )。

To investigate the effects of all trans retinoic acid (ATRA) on adhesion of bone marrow stromal cells derived from acute lympoblastic leukemia. Flow cytometry was used to detect the expression of adhesion molecules ICAM 1 and VCAM 1 on bone marrow stromal cells, MTT method was used to perform the adhesion assay of bone marrow stromal cells to normal hematopoietic cells or to tumor cells(Raji cells). The expression of ICAM 1 on ALL bone marrow stromal cells was enhanced by pharmacological concentration of ATRA (1 0 μmol/L)(P<0 05), the adhesion rates of bone marrow stromal cells to normal hematopoietic cells or to Raji cells were increased apparently by pharmacological concentration of ATRA (1 0 μmol/L)(P<0 05). [Conclusion] Pharmacological concentration of ATRA can enhance the adhesion capacities of ALL bone marrow stromal cells to normal hematopoietic cells and to tumor cells.