毛细管气相色谱法测定蜂蜡素及其胶囊中三十烷醇和二十八烷醇的含量

Determination of Triacontanol and Octacosanol in Fenglasu and Fenglasu Capsulas by Capillary Gas Chromatography

目的:建立蜂蜡素及其胶囊中三十烷醇和二十八烷醇毛细管气相色谱含量测定方法。方法:以氯仿为溶剂,三十六烷醇为内标物,采用SIMPLCITY-1毛细管柱(15m×0.53mm,1.5μm),FID检测器,载气为高纯氮气,流速27ml/min,进样口温度300℃,程序升温,初始温度240℃,维持1min后,以5℃/min升温至300℃,维持15min,结果:三十烷醉在0.4～1.6mg/ml;二十八烷醇在0.2～0.6mg/ml浓度范围内呈良好的线性关系(r_(30烷醇)=0.999,r_(28烷醇)=0.9996),三十烷醇的平均回收率为99.2％,RSD=1.15％;二十八烷醇的平均回收率为99.91％,RSD=1.40％(n=9)。结论:本法较准确,重现性较好,可用于蜂蜡素及其胶囊剂的质量监控。

Objective: The method was established for the determination of triacontanol and octacosanol in fenglasu and fenglasu capsulas.Methods:The solvent was chloroform. The internal standard was hexatriacontanol.Using SLMPLCITY-1 capillary column,flame ioniza-tion detector was specified, injector temperature was 300℃, and the column temperature rise by programming. [ Initial temperature was 240℃, maintaining 1 min and then rising the temperature (5℃·min-1) to 300℃for 15 min]. The flow rate of Nitrogen was 27 ml·min-1 .Results :The calibration curves were linear in the range of 0.4-1.6 mg/ml for triacontanol(r=0.999),0.2-0.6 mg/ml for octacosanol(r=0.9996)re-spectively.The average recovery(n=9) was 99.2% for triacontanol(RSD = 1.15%),99.91% for octacosanol(RSD = 1.40%). Conclusion: The method is rapid, accurate and reliable for the quality control of fenglasu and fenglasu capsulas.