人肺癌组织中单羧酸转运泵基因mRNA表达的变化

Change of monocarboxylate transporter gene mRNA expression in human lung cancer tissue

本文应用RT PCR方法扩增了人正常肺组织、肺癌组织中的MCT1、MCT2及 β actin基因mRNA ,以人 β actin基因RT PCR产物作为外参照 ,通过比较同一样本组中及样本组间MCT1、MCT2相对于 β actin的RT PCR产物的光密度比值 ,来分析判断MCT1、MCT2mRNA表达的高低。结果显示 ,MCT1在人正常肺组织、肺癌组织中的mRNA表达均高于MCT2 ;MCT1、MCT2基因mRNA在人肺癌组织中的表达明显高于癌旁正常肺组织。

The mRNA of MCT1,MCT2 and β actin in normal lung tissue and lung cancer tissue were amplified by RT PCR method; mRNA expression of MCT1 and MCT2 in tissue samples was semi quantitatively analyzed by comparing the relative ratio of MCT1 and MCT2 RT PCR product's OD ( optical density) vs.β actin RT PCR product's OD (β actin as an extecnae standard) . The results show that mRNA expression of MCT1 is significant higher than MCT2 in human normal lung tissue and lung cancer tissue; In human lung cancer tissue, the mRNA expression of MCT1 and MCT2 is significant higher than in that in the human normal lung tissue. Our data indicated that MCT1 might play an important role in intracellular pH regulation of tumor cells.