苯巴比妥对癫痫大鼠空间学习和海马一氧化氮合酶表达的影响

Effect of phenobarbital on spatial learning and hippocampal nNOS expression in epileptic rats

为研究苯巴比妥 (PB)对癫痫大鼠认知功能的影响和一氧化氮 (NO)信号通路的参与作用。以海人藻酸 (KA)致痫模型为研究对象 ,采用水迷宫测验、选择性神经型一氧化氮合酶 (nNOS)抑制剂— 7 硝基吲唑 (7 NI)、RT PCR技术观察PB对癫痫大鼠空间学习和海马nNOSmRNA表达的影响。结果显示 ,PB(6 0mg kgip )有确切的抗癫痫作用。PB对未致痫大鼠空间认知功能无明显影响 ,但可加重癫痫大鼠空间认知功能的损害。PB和KA均有增加大鼠海马nNOSmRNA表达作用 ,两者有协同作用。小剂量 7 NI(10mg kgip )可逆转PB(6 0mg kgip )增加大鼠海马nNOSmRNA表达的作用和对认知功能的影响。实验结果表明 。

To study the effects of phenobarbital (PB) on cognition in epileptic rats and involvement of NO signal pathway We investigated the effects of PB on spatial learning and the expression of nNOS mRNA in kainic acid (KA) rat model by Water maze test and RT PCR, respectively PB (60mg/kg, ip ) had exactly antiepileptic effects on KA treated rats and no effect on the spatial cognition in the normal rats But the KA treated rats after PB administration showed more severe cognitive dysfunction than those without PB injection The hippocampal nNOS mRNA expression was increased in rats with PB or KA treated PB and KA had synergetic effects on nNOS mRNA expression Lower dosage of 7 NI (10mg/kg,ip ) could decrease the nNOS mRNA lever and attenuate effectively cognitive disorders by PB (60mg/kg, ip ) in the rats with seizures NO signal pathway was involved the effects of PB on cognitive function in KA model