结肠直肠癌组织基质金属蛋白酶-2、-9表达水平与肿瘤血管形成的关系

Relationship between Expression of Matrix Metalloproteinase-2,Matri x Metalloproteinase－９and Angiogenesis in Colorectal Cancer

目的:探讨结肠直肠癌组织基质金属蛋白酶-2(matrixmetalloproteinase2,MMP2)和基质金属蛋白酶-9(matrixmetalloproteinase9,MMP9)的表达与肿瘤血管形成的关系。方法:应用免疫组化LSAB法进行研究,抗人MMP2、抗人MMP9和抗人CD31单克隆抗体分别用于检测72例结肠直肠癌手术切除标本MMP2、MMP9的表达情况及肿瘤微血管密度(microvesseldensity,MVD)。结果:72例结肠直肠癌组织中MMP2、MMP9的表达阳性率分别为69.4%和73.6%,DukesA、B、C、D各期的MMP2、MMP9的表达阳性率有递增的趋势(P值分别为0.013和0.027)。肿瘤血管形成与Dukes病理分期也存在一定联系,预后较好的DukesA、B期的MVD值明显低于预后较差的DukesC、D各期(P值<0.001)。MMP2、MMP9的表达情况与肿瘤血管形成的程度显著相关,MMP2、MMP9的表达阳性者的MVD值明显高于MMP2、MMP9的表达阴性者(P值分别为0.033和0.043)。结论:MMP2、MMP9在结肠直肠癌的血管形成中起重要作用,MMP2、MMP9可通过促进肿瘤血管形成影响肿瘤的进展。

Objective：To investigate the expression of m atrix metalloproteinase－２（MMP－２）and matrix metalloproteinase－９（MMP－９ ）in colorectal cancer and their relationship with angiogenesis．Methods ：The e xpression of MMP－２and MMP－９in ７２sections of colo rectal adenocarcinoma and microvessel density（MVD）in the neoplasms were determined by LSAB technique of immu nohistochemical staining with monoclonal antibodies against MMP－２，MM P －９，and CD３１．Results：The positive rates of the expression of MMP－２and MM P－９in ７２sections were６９．４％and ７３．６％，respectively．The positive ra tes of MMP－２and MMP－９were increased according to Duke' s stage（P values wer e ０．０１３and０．０２７，respectively）．Angiogenesis was also related with Du kes stage．MVD was significant hi gher in later stage than in earlier stage（P ＜０．００１）．Both the expression of MMP－２and MMP －９were correlated with a ngiogenesis．MVD in MMP－２or MMP－９positive samples was higher than those with negative expression（P values were ０．０３３and ０．０４３，respectively）．Co nclusions ：Both MMP－２and MMP－９are important fo r angiogenesis in colorectal adenocarcinoma．MMP－２and MMP－９may affect the progression of tumor b y promo tion of angiogenesis．