1,25-二羟基维生素D_3对环磷酰胺处理的NOD小鼠糖尿病的影响

Effect of 1,25-dihydroxyvitamin D_3 on cyclophosphamide induced diabetes in NOD mice

目的 探讨 1,2 5 -二羟基维生素 D3 对环磷酰胺促发的 NOD鼠 1型糖尿病的预防作用。方法 N OD鼠从离乳后隔日接受 1,2 5 -二羟基维生素 D3 治疗 ,第 10周龄给予环磷酰胺 30 0 m g/ kg,观察 1,2 5 -二羟基维生素 D3 对环磷酰胺处理的 NOD鼠糖尿病发病率和胰岛炎的影响 ,及对 Th1、Th2细胞因子 m RNA表达的影响。结果 1,2 5 -二羟基维生素 D3 处理组糖尿病发病率为 17% ,明显低于对照组 6 7% (P<0 .0 5 ) ,且胰岛炎严重程度也明显减轻。处理组胰腺 TNT- α、IFN- γ m RNA的表达较对照组明显降低 ,而 IL- 10 m RNA的表达无明显改变。结论 1,2 5 -二羟基维生素 D3 可以预防 NOD鼠环磷酰胺诱发的糖尿病的发生 ,其机制可能与纠正 Th1型细胞因子与

Objective To study the prophylactic effects of 1,25 dihydroxyvitamin D 3 on type 1 diabetes.Methods NOD mice received 1,25 dihydroxyvitamin D 3 (5 μg/kg) intraperitoneally every other day from the time of weaning,inducing diabetes by cyclophosphamide (300 mg/kg) at 70 days old to observe the incidence of diabetes and the degree of insulitis.RT PCR was performed for TNF α,IFN γ and IL 10.Results The incidence of diabetes in the 1,25 dihydroxyvitamin D 3 group was 17% which was obviously less than 67% in control group (P<0.05),and the degree of insulitis in the test group was also less than in the control group.The author observed an decrease in pancreatic mRNA levels of TNF α and IFN γ.No differences in pancreatic mRNA level of IL 10 was seen between control and test mice.Conclusion 1,25 Dihydroxyvitamin D 3 can prevent NOD mice from developing type 1 diatetes,probably through regulating Th1/Th2 imbalance.