尿道粘膜上皮细胞的分离培养和鉴定

The study of urethral epithelium culture in vitro

目的 为采用组织工程技术构建尿道粘膜组织提供实验依据。 方法 取雄性新西兰幼兔尿道粘膜组织小块 ,酶消化成单细胞悬液 ,接种后静置培养、传代。动态观察细胞形态变化及生长增殖情况 ,细胞进行常规组化染色、免疫组化染色及流式细胞仪检查 ,并观察超微结构。 结果 整个上皮细胞生长期内无成纤维细胞混杂生长 ,均为单一的上皮细胞 ,并证实为二倍体细胞。细胞可传 11～ 13代 ,成活 5 0～ 6 0d。 结论 新西兰幼兔尿道粘膜上皮细胞可在体外培养 ,在一定时间内保持增殖能力。

Objective To study urethral epithelium cu lt ure in vitro as a basic role in the further study on tissue engineering urethra. Methods A bit of urethral mucosa was taken from a young male New Zealand hare and was digested by enzyme into single cell liquid.The ce ll was then cultured in a still condition.The medium was renewed regularly and t he cells were subcultured and studied. Results All the c utured cells were urethral diploid cells without fibroblasts.The cells could be subcultured 11～13 generations with a surviving period of 50～60 days. Conclusions The urethral epithelium the cultured cells of young N ew Zealand hare can be cultured in vitro and is able to proliferate within a cer tain period.This provides a strong foundation for the further study on tissue en gineering urethra.