汉防己甲素对大鼠脊髓损伤氧自由基的影响

The effect of tetrandrine on free radicals of spinal cord injury in rats

目的观察汉防己甲素（Ｔｅｔ）对急性脊髓损伤（ＡＳＣＩ）的保护作用，并从自由基损伤的角度探讨其对ＡＳＣＩ的作用机理。方法７５只 Ｗｉｓｔａｒ大鼠改良Ａｌｉｅｎ’ｓ打击法复制大鼠全瘫模型后随机分为 ３组：（１）生理盐水（ＮＳ）组；（２）汉防己甲素（Ｔｅｔ）组；（３）甲基强的松龙组（ＭＰ）。伤后 ３０分钟 ｉｖ Ｔｅｔ２２．５ｍｇ／ｋｇ体重。于伤后１小时、４小时、８小时取损伤区脊髓组织进行形态学观察和生化指标测定。结果汉防己甲素能明显降低组织钙、ＭＤＡ含量，防止ＳＯＤ含量的下降，使伤区脊髓组织中水含量减少，防止ＬＤＨ、ＣＰＫ等细胞内物质的泄漏，减轻组织病损。结论Ｔｅｔ对ＡＳＣＩ有保护作用，这一作用机制是通过抑制自白基的生成、减轻脂质过氧化的程度并与其钙拮抗起协同治疗作用。

Objective To study the protective effect of tetrandrine (Tet) on free radicals of acute spinal cord injury (ASCI) in rats and activity mechanism to ASCI from be radicals. Methods 75 Wistar rats (male and female) were randomly divided into three groups. All experimental rats injuried by 100g. cm force were resulted in paralegia by modified Alien's technique. Tet was administered at 30 minutes posttraumatically. 1, 4, 8 hours posttraumatically, samples of spinal cord were taken out to assay calcium concentration and content of MDA, SOD H_2O. Pathological changes of spinal cord were observed. Results ASCI, Tet can decrease significantly calcium concentration and content of MDA and H_2O increase content of SOD after ASCF. The effects were similar with MP. Conclusion The results provided that tetrandrine has protective effects on contused of spinal cords in rats, which the mechanisms are due to the synergism of preventing from the production of reactive oxygen metablity, attenuating the injury of peroxidation and blocking the calcium channel.