铝胁迫对小麦根呼吸作用和一些线粒体结合酶活性影响

Effects of Aluminum on Respiratory Rate and Some Mitochondrial Enzymes Activit ies of Wheat Roots

比较了耐铝性不同的两品种小麦 Altas66和 Scout66根呼吸速率、线粒体 H+ - ATP酶、Ca2 + - ATP酶和焦磷酸酶活性变化。铝处理后 ,两品种小麦根呼吸速率均下降 ,随处理铝浓度增加而加剧 ,但对照和处理条件下 ,Altas 66根呼吸速率均高于 Scout 66。两品种小麦根线粒体膜上都存在H+ - ATP酶、Ca2 + - ATP酶和 H+ - PP酶 ,离体和活体铝处理后 ,这些酶活性受到抑制 ,在活体处理和Scout66中更为明显。

The changes in respiratory rate and in mitochondrial H + ATPase, Ca 2+ ATPase and H + PPase activities from the roots of two wheat ( Triticum aest iv um L.)cultivars, Altas 66 (Al tolerant)and Scout 66 (Al sensitive)were compa red un der aluminum stress. Aluminum treatment inhibited the respiratory rate of two wh eat cultivars roots. It was more distinct as the aluminum concentration increase d, and the respiratory rate of Scout 66 was higher than that of Altas 66 under t he control and the treatment. The activities of mitochondrial H + ATPase, Ca 2+ ATP ase and H + PPase of two wheat cultivars roots were inhibited by aluminum trea tment in vivo and in vitro, and they were more serious in vivo treat ment and in Scout 66 than those in vitro treatment and in Altas 66. The decr ease of respiratory rate may be related to the inhibition of mitochondrial enzym es.