“黄白散”对兔接种RHD疫苗后免疫功能的影响

Effect of "yellow white powder"on immune function of rabbit after inoculation with RHD vaccine

在注射兔病毒性出血症 (RHD)疫苗后 ,30只兔随机分为 2组 ,试验组兔饲喂含 1 5 %黄白散的饲料。从接种到第 1 80天 ,每组取兔 8只 ,每隔 1 5d采血 1次 ,检测血凝抑制 (HI)抗体 ;在接种前 1天及接种后 5、 1 0、 1 7、 2 4、 31、 41、 5 1d ,每组取兔 1 0只 ,采血检测ANAE+淋巴细胞百分率 ;在 1 80、 2 4 0、 30 0d时 ,每组分别取兔 5只做攻毒保护试验。结果表明 :2组兔HI抗体峰值分别为 7 3log2和 9 8log2 ,差异极显著 (P <0 0 1 ) ;试验组疫苗保护期可延长 60d ;试验组ANAE+率从第 1 0天至 5 1天与对照组比较 ,差异极显著(P<0 0 1 )。所以黄白散可增强兔接种RHD疫苗后的特异性免疫功能 ,增强RHD疫苗的免疫效果。

Rabbits( n= 30)inoculated with rabbit virus hemorrhage disease(RHD)vaccine were randomly divided into two groups.Diets of rabbits in treatment contained 1 5% of'yellow white powder'.Blood samples from treatment and control groups were taken for assay of HI antibody once 15 days from 0 to 180 d after inoculation and for determining ANAE + percentage of lymphocytes from 1d before vaccination to 5,10,17,24,31,41 and 51d after vaccination.5 of rabbits in each group were inoculated with RHD at 180,240,and 300 d after vaccination.The results indicated that the peak values of HI antibodies in two group were 9 8 log2 and 7 31 log2,respectively( P <0 01).Immunization period in the treatment was prolonged for 60 days,ANAE + rate in the treatment from 10 to 51 d after vaccination was more than that in control( P <0 01).'Yellow white powder'could enhance the specific immune function of rabbit inoculated with RHD vaccine,and increase the immune effect of RHD vaccine.