褐藻酸降解菌感染对海带抗氧化酶活性的影响

Effects of infection by algnic acid decomposing bacteria on activity of antioxidant enzymes in Laminaria japonica

利用生物化学的方法，研究了海带被褐藻酸降解菌感染过程中的抗氧化酶活性的变化。结果表明，（１）在褐藻酸降解菌感染的初期，海带细胞内超氧化物歧化酶（ＳＯＤ）和过氧化氢酶（ＣＡＴ）的活性急剧升高，随着感染的进行，二者的活性逐渐下降。（２）抗坏血酸过氧化物酶（ＡＰＸ）的活性在感染的初期变化不明显，而在感染的后期活性有所增加。（３）在整个感染的过程中，谷胱甘肽过氧化物酶（ＧＰＸ）的活性的变化无规律性，没有表现出明显的升降变化。说明这四种酶在海带抵抗褐藻酸降解菌感染的过程中所起作用的大小和作用的时期有所差异．

Changes of antioxidant enzyme activity in Laminaria japonica during infection process caused by algnic acid decomposing bacteria were studied using biochemical methods. The results showed as follows: (1) The activity of superoxide dismutase (SOD) and catalase (CAT) increased sharply at the first stage of infection and decreased gradually with the further infection. (2) The activity of ascorbic acid peroxidase (APX) showed no remarkable change in the initial infection stage, but increased during later stage of infection. (3) The activity of glutathione peroxidase( GPX) exhibited irregular changes during whole infection process.