虾池环境生物修复作用菌生长影响因子的研究

Study of factors influencing the growth of functional bacteria in bioremediation of shrimp culture environment

采用吖啶橙荧光染色计数法和吸光度法，测定了不同温度、盐度、初始ｐＨ、溶解氧、接种量及不同培养基配方对虾池环境生物修复作用菌生长的影响。结果表明，大部分作用菌在１６－４０℃、盐度０－４０、ｐＨ６．６－９．０的范围内有氧或微氧条件下均可生长，能适应一般虾池的环境条件，培养基中磷酸盐是必不可少的。在２８－３４℃、盐度２０－３０、ｐＨ７．４－８．２的有氧条件下生长最好；可作为大规模生产时的培养条件。为增加菌体产量和活力，可适当增加接种量，提高蛋白陈和酵母膏的浓度至２２１６Ｅ配方浓度的五倍，培养时间以１８－２０ｈ为宜．

Influences of temperature, salinity, pH, soluble oxygen, inoculation volume and composition of medium on the growth of functional bacteria in bioremediation of shrimp culture environment were studied with acridine orange direct count (AODC) and light absorption methods. The results indicated that most functional bacteria strains can grow in aerobic or semi-aerobic condition at 16-40℃,salinity 0-40 and pH 6.6-9.0. Phosphate was indispensable for growth and most shrimp ponds can meet this condition. 28- 34℃, salinity 20- 30, pH 7.4- 8.2 with rich soluble oxygen can be used as culture conditions in large-scale bacteria production, and the concentration of peptone, yeast extract should be raised 5 times and inoculation volume may increase properly. To increase the amount of bacteria, best time period for culture is 18-20h.