摘要
采用PCR方法,追踪养殖鳜的亲鱼、子代、饵料鱼及与鳜养殖环境相关的水体生物和非生物因子中鳜鱼病毒的存在状况,并探讨鳜鱼病毒的传播途径。本研究中,鳜鱼塘水体中采集的除鳜以外的其他生物、底泥及水样的核酸抽提样本PCR扩增病毒结果呈阴性;感染病毒的亲鱼,其性腺中可检测出病毒,其子代组织病毒检测亦呈阳性。研究获得病毒可以在鳜体内呈潜伏状态存在和病毒可以垂直传播的一些证据。
In order to discuss the transmission of Spercara chuatsi virus (SCV), the existence of SCV was checked in the tissues of parent fish, their offspring, feedstuff fish, and in some factors relating to rearing environment of mandarin fish Spercara chuatsi by means of polymerase chain reaction (PCR). The SCV positive results were demonstrated in the parents' gonad and their offspring, while the SCV negative results were shown in all other samples collected in this experiment, including the diseased pond' s environmental factors such as plankton, upper-layer silt, and some aquatic insects. Some evidences were acquired in this investigation that SCV can exist in Spercara chuatsi in a latent state and can transmit from parent to its offspring.
出处
《水产学报》
CAS
CSCD
北大核心
2001年第5期460-463,共4页
Journal of Fisheries of China
基金
广东省自然科学基金资助(980907)
广东省海洋与渔业局资助"池塘水生态与鳜鱼病害关系及调控技术的研究"
关键词
鳜鱼病毒
聚合酶链式反应
传播途径
鱼病
Spercara chuatsi virus (SCV)
polymerase chain reaction
transmission route