传染性脾肾坏死病毒、ConA和LPS对体外鳜的头肾淋巴细胞转化的影响

In vitro influences of infectious spleen and kidney necrosis virus (ISKNV) , ConA and LPS on the pronephric lymphocytes from Siniperca chuatsi

采用 ＷＴＴ颜色反应法研究传染性脾肾坏死病毒（ＩＳＫＮＶ）、伴刀豆球蛋白 Ａ（ｃｏｎｃａｎａｖａｌｌｉｎ Ａ，ＣｏｎＡ）和脂多糖（ｌｉｐｏｐｏｌｙｓａｃｃｈａｒｉｄｅ，ＬＰＳ）在离体状态下对健康和ＩＳＫＮＶ感染后存活３０ｄ或６０ｄ的鳜的头肾淋巴细胞转化的影响。结果表明，ＣｏｎＡ和ＬＰＳ能促进健康级头肾淋巴细胞的转化，而对感染后存活３０ｄ或６０ｄ的鳜头肾淋巴细胞没有作用；纯化的ＩＳＫＮＶ对健康鳜头肾淋巴细胞没有促进淋巴细胞转化的作用，但对ＣｏｎＡ的作用有抑制，对感染后存活的３０ｄ或６０ｄ的鳜头肾淋巴细胞，纯化的ＩＳＫＮＶ有一定的促进转化的作用。感染存活的鳜头肾中有对ＩＳＫＮＶ的免疫记忆性Ｔ细胞。另外，用建立的ＩＳＫＮＶ ＰＣＲ检测方法对健康和ＩＳＫＮＶ感染后存活的鳜组织进行了检测，结果显示，健康鳜为阴性，而ＩＳＫＮＶ感染后存活的鱼，头肾、后肾、脾脏和部分鱼的心脏为阳性。ＩＳＷ在鳜体内形成潜伏感染。

In vitro, the influences of JSKNV, concanavallin A (ConA) and lipopolysaccharide (LPS) on the pronephric lymphocytes from healthy mandarin fish Siniperca chuatsi and survivors for 30d or 60d infected from JSKNV were determined by the MTT colorimetric assay method. The results showed that the pronephric lymphocytes from healthy mandarin fish proliferated in response to ConA and LPS, not to ISKNV. However, ISKNV were effective to suppress the function of ConA. The pronephric lymphocytes from mandarin fish surviving for 30d or 60d from ISKNV did not proliferate in response to ConA and LPS, and were susceptible to ISKNV. The T cell may exist in pronephron. In addition, the healthy mandarin fish were negative and the fish surviving from ISKNV 30d or 60d were positive in the tissues of spleen, kidney and heart in part by PCR assays for ISKNV DNA. ISKNV was latent in survivors.