结合SADF与RAPD标记构建家蚕连锁图

Construction of a linkage map in the silkworm, Bombyx mori, based on SADF and RAPD markers

用 4 0个选择性扩增多态性引物和 137个随机性扩增多态性引物对家蚕BombyxmoriF2 群体进行分子标记的筛选。获得 5 4 4个符合遗传分离比的多态性位点 ,并用Mapmaker软件进行连锁分析。在最小LOD值为 4 ,最大重组值为 0 2条件下拆分连锁群 ,并对处于同一连锁群的位点进行合理排序 ,计算出位点间的重组值后转换为图谱距离 ,构建了一个家蚕的分子连锁图。

DNA markers which were tested for the expected 3∶1 segregation by the chi-square test for 69 F 2 individuals were detected with 40 SADF synthetic primers and 137 RAPD primers. Using these DNA markers the linkage analysis was performed by Mapmaker/Exp (Version 3 0b). The following parameters were preset: two-point analysis (LOD 3 0, maximum recombination value 0 3); three-point and multipoint analysis (LOD 3 0, maximum recombination value no more than 0 2). Linked markers were also identified at the “group” command with LOD score 4 and maximum recombination value 0 2 The functions of Mapmaker were employed to assess the order of the markers in each group. Recombination values were converted into map distance (centimorgan, cM) by applying the Kosambi function. A molecular linkage map of silkworm was constructed.